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中文名稱： 兔抗PRKAR1A多克隆抗體
英文名稱： Anti-PRKAR1A rabbit polyclonal antibody
別 名： PRKAR1A; CAR; CNC; CNC1; DKFZp779L0468; MGC17251; PKR1; PPNAD1; PRKAR1; TSE1
相關(guān)類別： 一抗
儲(chǔ) 存： 冷凍（-20℃） 避光
宿 主： Rabbit
抗 原： PRKAR1A
反應(yīng)種屬： Human, Mouse, Rat
標(biāo) 記 物： Unconjugate
克隆類型： rabbit polyclonal
技術(shù)規(guī)格
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Background: |
The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197. |
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Applications: |
WB |
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Name of antibody: |
PRKAR1A |
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Immunogen: |
Fusion protein of human PRKAR1A |
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Full name: |
protein kinase, cAMP-dependent, regulatory, type I, alpha |
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Synonyms： |
PRKAR1A; CAR; CNC; CNC1; DKFZp779L0468; MGC17251; PKR1; PPNAD1; PRKAR1; TSE1 |
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SwissProt: |
P10644 |
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WB Predicted band size: |
43 kDa |
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WB Positive control: |
HeLa, CEM, A549, SW480, PC3, V251, HCTnb and heart tissue |
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WB Recommended dilution: |
500-2000 |
